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Lianhuaqingwen exerts anti-viral and anti-inflammatory activity against novel coronavirus (SARS-CoV-2)

Li Runfeng;Hou Yunlong;Huang Jicheng;Pan Weiqi;Ma Qinhai;Shi Yongxia;Li Chufang;Zhao Jin;Jia Zhenhua;Jiang Haiming;Zheng Kui;Huang Shuxiang;Dai Jun;Li Xiaobo;Hou Xiaotao;Wang Lin;南山 钟;Yang Zifeng

Guangzhou Medical College;Hebei University of Chinese Medicine;Technology Centre;KingMed Virology Diagnostic & Translational Center;China Association for Science and Technology;Macau University of Science and Technology

发表时间:2020-6

期 刊:Pharmacological Research

语 言:English

U R L: http://www.scopus.com/inward/record.url?scp=85083017858&partnerID=8YFLogxK

摘要

Purpose: Lianhuaqingwen (LH) as traditional Chinese medicine (TCM) formula has been used to treat influenza and exerted broad-spectrum antiviral effects on a series of influenza viruses and immune regulatory effects Ding et al. (2017). The goal of this study is to demonstrate the antiviral activity of LH against the novel SARS-CoV-2 virus and its potential effect in regulating host immune response. Methods: The antiviral activity of LH against SARS-CoV-2 was assessed in Vero E6 cells using CPE and plaque reduction assay. The effect of LH on virion morphology was visualized under transmission electron microscope. Pro-inflammatory cytokine expression levels upon SARS-CoV-2 infection in Huh-7 cells were measured by real-time quantitative PCR assays. Results: LH significantly inhibited SARS-CoV-2 replication in Vero E6 cells and markedly reduced pro-inflammatory cytokines (TNF-α, IL-6, CCL-2/MCP-1 and CXCL-10/IP-10) production at the mRNA levels. Furthermore, LH treatment resulted in abnormal particle morphology of virion in cells. Conclusions: LH significantly inhibits the SARS-COV-2 replication, affects virus morphology and exerts anti-inflammatory activity in vitro. These findings indicate that LH protects against the virus attack, making its use a novel strategy for controlling the COVID-19 disease.

关键词

Anti-inflammatory
Coronavirus
Lianhuaqingwen
SARS-CoV-2

相关科学

药理学,毒理学和制药学
药理学

文献指纹

医学与生命科学

lianhuaqingwen

SARS Virus

Coronavirus

Anti-Inflammatory Agents

Antiviral Agents

Vero Cells

Virion

Viruses

Cytokines

Chinese Traditional Medicine

Orthomyxoviridae

Virus Replication

Human Influenza

Real-Time Polymerase Chain Reaction

Electrons

Interleukin-6

Messenger RNA

Infection

In Vitro Techniques

Therapeutics

被引量

期刊度量

Scopus度量

年份 CiteScore SJR SNIP
1996
1997
1998
1999 0.308 0.624
2000 0.372 0.554
2001 0.437 0.594
2002 0.499 0.73
2003 0.528 0.736
2004 0.649 0.803
2005 0.805 0.988
2006 0.992 1.081
2007 0.986 1.129
2008 1.191 1.176
2009 1.333 1.135
2010 1.479 1.397
2011 6.6 1.488 1.394
2012 7.8 1.493 1.637
2013 9 1.702 1.438
2014 9 2.031 1.328
2015 8.5 2.143 1.529
2016 7 2.037 1.362
2017 7.4 1.811 1.268
2018 8.9 1.781 1.433
2019 9.9 1.609 1.499
2020 9.9
2021

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